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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION

《农业科学与工程前沿（英文）》 2021年 第8卷 第2期

摘要：

Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

 

关键词： Citrus / 2n gamete / interploidy hybridization / pericentromeric SNP marker / second division restitution    

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UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

《农业科学与工程前沿（英文）》   页码 302-313 doi: 10.15302/J-FASE-2021385

摘要： Seedless fruits are desirable in the citrus fresh fruit market. Triploid production via diploid × tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus. Although ‘Orah’ mandarin has desirable organoleptic qualities, seeds in the fruits weaken its market competitiveness. To produce new seedless cultivars that are similar to ‘Orah’ mandarin, we performed three 2x × 4x crosses using ‘Orah’ mandarin as the seed parent to regenerate triploid plantlets. A total of 182 triploid and 36 tetraploid plantlets were obtained. By analyzing their genetic origins using nine novel single nucleotide polymorphism (SNP) markers, all of the triploids and tetraploids derived from these three crosses were proven to be hybrids. Also, we demonstrated that 2n megagametophyte formation in ‘Orah’ mandarin result in tetraploid production in these three interploidy crosses. These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers. Based on the genotypes of the 2n megagametophytes, the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%, indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels. These triploid hybrids enrich the germplasm available for seedless breeding. Moreover, the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits.

关键词： Citrus     2n gamete     interploidy hybridization     pericentromeric SNP marker     second division restitution    

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High-order phase-field model with the local and second-order max-entropy approximants

Fatemeh AMIRI

《结构与土木工程前沿（英文）》 2019年 第13卷 第2期   页码 406-416 doi: 10.1007/s11709-018-0475-5

摘要： We approximate the fracture surface energy functional based on phase-field method with smooth local maximum entropy (LME) and second-order maximum entropy (SME) approximants. The higher-order continuity of the meshfree methods such as LME and SME approximants allows to directly solve the fourth-order phase-field equations without splitting the fourth-order differential equation into two second-order differential equations. We will first show that the crack surface functional can be captured more accurately in the fourth-order model with smooth approximants such as LME, SME and B-spline. Furthermore, smaller length scale parameter is needed for the fourth-order model to approximate the energy functional. We also study SME approximants and drive the formulations. The proposed meshfree fourth-order phase-field formulation show more stable results for SME compared to LME meshfree methods.

关键词： second-order maximum entropy     local maximum entropy     second- and fourth-order phase-field models     B-spline    

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Numerical simulation of sudden-expansion two-phase flows with two-scale second-order moment particle

ZENG Zhuoxiong, HAN Shoulei, ZHOU Lixing

《能源前沿（英文）》 2007年 第1卷 第2期   页码 202-206 doi: 10.1007/s11708-007-0026-6

摘要： A two-scale second-order moment particle turbulence model is developed, based on the concept of particle large-scale fluctuation due to turbulence and particle small-scale fluctuation due to collision. The model is employed to simulate gas-particle flows in a sudden-expansion chamber. Simulation results are compared with the experimental results and with those obtained by the single-scale second-order moment two-phase turbulence model. It is shown that the two-scale model is with higher calculating accuracy than the single-scale model.

关键词： gas-particle     single-scale second-order     large-scale fluctuation     second-order     sudden-expansion    

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Control division of agricultural non-point source pollution at medium-sized watershed scale in Southeast

HUANG Jinliang, HONG Huasheng, ZHANG Luoping

《环境科学与工程前沿（英文）》 2008年 第2卷 第3期   页码 333-339 doi: 10.1007/s11783-008-0035-7

摘要： This paper presents the study carried out for controlling agricultural non-point source pollution (NSP) in a medium-sized watershed covering 1.47 × 10 km in Southeast China using quantitative analysis coupled with geographic information system (GIS), universal soil loss equation (USLE), soil conservation service-curve number (SCS-CN), nutrient loss equations, and annualized agricultural nonpoint source model (AnnAGNPS). Based on the quantitative results derived from GIS and environmental models, five control division units were generated for NSP control in Jiulong River watershed, namely, controlling unit for soil losses, controlling unit for livestock breeding and soil losses, controlling unit for excessive fertilizer use and livestock breeding, controlling unit for soil losses and fertilizer use, and controlling unit for excessive fertilizer use and soil losses. This study proved that integrating GIS with environmental models can be adopted to efficiently evaluate major sources and contributors of NSP, and identify the critical source areas of NSP, which enables adjusting measures to local conditions by further control division units developed through such study for control and management of water quality degradation induced by NSP in the Jiulong River watershed.
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Impact analytical models for earthquake-induced pounding simulation

Kun YE, Li LI

《结构与土木工程前沿（英文）》 2009年 第3卷 第2期   页码 142-147 doi: 10.1007/s11709-009-0029-y

摘要： Structural pounding under earthquake has been recently extensively investigated using various impact analytical models. In this paper, a brief review on the commonly used impact analytical models is conducted. Based on this review, the formula used to determine the damping constant related to the impact spring stiffness, coefficient of restitution, and relative approaching velocity in the Hertz model with nonlinear damping is found to be incorrect. To correct this error, a more accurate approximating formula for the damping constant is theoretically derived and numerically verified. At the same time, a modified Kelvin impact model, which can reasonably account for the physical nature of pounding and conveniently implemented in the earthquake-induced pounding simulation of structural engineering is proposed.

关键词： structural pounding     Hertz model     Kelvin model     nonlinear damping     coefficient of restitution    

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SUSTAINABLE PLANT PEST MANAGEMENT THROUGH OPTIMIZATION AND MINIMIZATION

Plant Production and Protection Division (NSP), Food and Agriculture Organization of the United Nations

《农业科学与工程前沿（英文）》 2022年 第9卷 第1期   页码 161-166 doi: 10.15302/J-FASE-2021426

摘要：

Plant pests and diseases have significant negative impacts on global food security, world trade and rural livelihoods. Climate change exacerbates these impacts in certain parts of the world. Overreliance on pesticides as the primary tool for plant pest management leads to problems such as pesticide resistance and pest resurgence. Environmental and food safety concerns are also associated with overuse of pesticides in crop production. There is clearly a need for a shift in pest management strategies and practices globally. Optimization of structures and functions in crop production agroecosystems through soil conservation practices and cropping diversification can improve pest regulation services provided in the systems. Prioritization of safer alternatives and practices in the IPM pyramid, such as resistant varieties and biopesticides, helps minimize the use of potentially risky agricultural inputs such as synthetic pesticides. Investment is needed to boost the development of innovative green technologies and practices. Production, distribution, use and regulatory capacities need to be strengthened to facilitate large-scale adoption of green technologies and practices. Finally, policy, financial and market instruments should be wielded to provide an enabling environment for the transformation to sustainable plant pest and disease management strategies and practices worldwide.

 

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What should be done for the “second half” of China’s urbanization?

Chunfei WU, Xiaojiang LI, Pengfei LI

《工程管理前沿（英文）》 2018年 第5卷 第2期   页码 276-277 doi: 10.15302/J-FEM-2017106

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Emissions analysis on second generation biodiesel

Yuvarajan Devarajan,Ravi kumar Jayabal,Devanathan Ragupathy,Harish Venu

《环境科学与工程前沿（英文）》 2017年 第11卷 第1期 doi: 10.1007/s11783-017-0891-0

摘要： CNSBD can be used as a fuel without any modification in existing CI engine. Pentanol reduces HC, CO, NO and Smoke emissions significantly. New and next generation alternative fuel is employed. Blending of CNSBD with pentanol resulted in no separation of phases and found miscible. This work investigates the effect of adding pentanol with biodiesel derived from cashew nut shell on its emissions characteristics is conducted in stationery diesel engine. The main purpose of this work is intended to reduce the emissions by fuelling biodiesel derived from cashew nut shell and the pentanol blends. Cashew nut shell biodiesel is prepared by transesterification process. Oxygenated additive used in the work is Pentanol. The experiment is conducted using four test fuels such as, biodiesel derived from cashew nut shell (CNSBD), a fuel containing 90% cashew nut shell biodiesel and 10% pentanol (CNSBD90P10), a fuel containing 80% cashew nut shell biodiesel and 20% pentanol (CNSBD80P20) and neat diesel. Experimental work concluded that by adding 10% of pentanol to cashew nut shell biodiesel 10.1%, 2.6%, 5.1% and 2.1%reduction in CO, HC, NO and Smoke emissions were observed respectively. Further by fueling with these blends, no modifications in engines were required.

关键词： Pentanol     Biodiesel     Emissions    

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Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division

Claudia DOEL,Zhidong ZHANG,Lise MAZELET,Ryan WATERS,John BASHIRUDDIN

《农业科学与工程前沿（英文）》 2014年 第1卷 第3期   页码 250-257 doi: 10.15302/J-FASE-2014031

摘要： Foot-and-mouth-disease virus (FMDV) replicates in epithelial cells. The restriction of FMDV RNA to the basal cell layer of epithelia suggests a possible link between FMDV replication and the cell status. This paper describes studies in which FMDV infection was investigated in cells that were held at various cell division phases using cell cycle inhibitors. The results suggest that when cells were arrested at the G or G /S phase, high levels of viral RNA were detected by quantitative real-time reverse transcription PCR and viral protein synthesis was observed by specific labeling techniques. In contrast, when cells were arrested at the G /M phase, reduced or no viral RNA synthesis was detected.

关键词： foot-and-mouth disease virus     cell cycle     replication    

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The second short-term warm ischemia after vascular anastomosis did not affect early renal function recovery

null

《医学前沿（英文）》 2012年 第6卷 第3期   页码 329-331 doi: 10.1007/s11684-012-0211-9

摘要：

Ischemic postconditioning was defined as rapid intermittent interruptions of blood ?ow in the early phase of reperfusion, which has been found to be protective against renal ischemia-reperfusion injury (IRI) in animal models but not in clinical trials. We describe a case that the allograft renal vein was twisted because of the surgeon’s mistake, which caused the warm ischemia of allograft after reperfusion. The allograft restored blood flow without second reperfusion and cold preservation after 9 min of warm ischemia. The patient was followed up for 3 months and the allograft worked well without complications.

关键词： renal transplantation     vein twist     ischemia-reperfusion injury    

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Second unmanipulated allogeneic transplantation could be used as a salvage option for patients with relapsed

《医学前沿（英文）》 2021年 第15卷 第5期   页码 728-739 doi: 10.1007/s11684-021-0833-x

摘要： Relapse is the main problem after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The outcome of a second allo-HSCT (HSCT2) for relapse post-HSCT has shown promising results in some previous studies. However, little is known about the efficacy of HSCT2 in patients with relapsed/refractory acute leukemia (AL) post-chemotherapy plus modified donor lymphocyte infusion (post-Chemo+m-DLI) after the first allo-HSCT (HSCT1). Therefore, we retrospectively analyzed the efficacy of HSCT2 in 28 patients with relapsed/refractory AL post-Chemo+m-DLI in our center. With a median follow-up of 918 (457–1732) days, 26 patients (92.9%) achieved complete remission, and 2 patients exhibited persistent disease. The probabilities of overall survival (OS) and disease-free survival (DFS) 1 year after HSCT2 were 25.0% and 21.4%, respectively. The cumulative incidences of nonrelapse mortality on day 100 and at 1 year post-HSCT2 were 7.1%±4.9% and 25.0%±8.4%. The cumulative incidences of relapse were 50.0%±9.8% and 53.5%±9.9% at 1 and 2 years post-HSCT2, respectively. Risk stratification prior to HSCT1 and percentage of blasts before HSCT2 were independent risk factors for OS post-HSCT2, and relapse within 6 months post-HSCT1 was an independent risk factor for DFS and relapse post-HSCT2. Our findings suggest that HSCT2 could be a salvage option for patients with relapsed AL post-Chemo+m-DLI.

关键词： second hematopoietic stem cell transplantation     acute leukemia     relapse     chemotherapy     modified donor lymphocyte infusion    

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Development of splenic surgery in China

JIANG Hongchi, ZHAO Xianqi, GAO Yue

《医学前沿（英文）》 2007年 第1卷 第2期   页码 126-129 doi: 10.1007/s11684-007-0024-4

摘要： Splenic surgery has enjoyed rapid progress for the past decades in China. Based on continuously solidifying theories and clinical practice, many advanced concepts with applicable clinical frameworks have been established, thus making splenic surgery an indispensable division in modern surgery. In this study, the authors try to give an overview of the developments in this field. Spleen function is a key issue that generally guides advances in splenic surgery. The old knowledge of the dispensable spleen has been abandoned, while the exact role that it plays in the body continues to be unveiled. Now, standard grading for splenic injury and the respective guidelines for clinical management have been established, with the use of selective instead of indiscriminate splenectomy being accepted by most experts. Various spleen sparing operations have been developed, while many advanced techniques such as laparoscopy have been introduced to splenic surgery. Spleen transplantation has greatly advanced in China, even though there are still many unsolved mysteries in this field. Altogether, splenic surgery is a new and prosperous discipline, and it is a tremendous stage for young surgeons to be part of.

关键词： function     respective     prosperous discipline     knowledge     indispensable division    

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分形方法导出改进的牛顿第二定律及万有引力定律

付昱华

《中国工程科学》 2003年 第5卷 第6期   页码 55-58

摘要：

牛顿第二定律及万有引力定律是根据实验结果总结出来的。为了探讨从理论上导出这两个定律的可能性，根据能量守恒定律，给出用变维分形方法针对一个实例（小球沿长斜面滚下）导出改进的牛顿第二定律及万有引力定律的方法。具体给出了适用于实例的常维分形结果：改进的万有引力定律F=-GMm / r1.99989和改进的牛顿第二定律F=ma1.01458。

关键词： 分形方法     牛顿第二定律     万有引力定律     理论推导    

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Comparison between linear and non-linear forms of pseudo-first-order and pseudo-second-order adsorption

Junxiong LIN , Lan WANG ,

《环境科学与工程前沿（英文）》 2009年 第3卷 第3期   页码 320-324 doi: 10.1007/s11783-009-0030-7

摘要： The best-fit equations of linear and non-linear forms of the two widely used kinetic models, namely pseudo-first-order and pseudo-second-order equations, were compared in this study. The experimental kinetics of methylene blue adsorption on activated carbon was used for this research. Both the correlation coefficient () and the normalized standard deviation Δ(%) were employed as error analysis methods to determine the best-fitting equations. The results show that the non-linear forms of pseudo-first-order and pseudo-second-order models were more suitable than the linear forms for fitting the experimental data. The experimental kinetics may have been distorted by linearization of the linear kinetic equations, and thus, the non-linear forms of kinetic equations should be primarily used to obtain the adsorption parameters. In addition, the Δ(%) method for error analysis may be better to determine the best-fitting model in this case.

关键词： adsorption     pseudo-first order     pseudo-second order     kinetic model     linear method     non-linear method    

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标题 作者 时间 类型 操作

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION

期刊论文

UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION

Qiangming XIA, Wei WANG, Kaidong XIE, Xiaomeng WU, Xiuxin DENG, Jude W. GROSSER, Wenwu GUO

期刊论文

High-order phase-field model with the local and second-order max-entropy approximants

Fatemeh AMIRI

期刊论文

Numerical simulation of sudden-expansion two-phase flows with two-scale second-order moment particle

ZENG Zhuoxiong, HAN Shoulei, ZHOU Lixing

期刊论文

Control division of agricultural non-point source pollution at medium-sized watershed scale in Southeast

HUANG Jinliang, HONG Huasheng, ZHANG Luoping

期刊论文

Impact analytical models for earthquake-induced pounding simulation

Kun YE, Li LI

期刊论文

SUSTAINABLE PLANT PEST MANAGEMENT THROUGH OPTIMIZATION AND MINIMIZATION

Plant Production and Protection Division (NSP), Food and Agriculture Organization of the United Nations

期刊论文

What should be done for the “second half” of China’s urbanization?

Chunfei WU, Xiaojiang LI, Pengfei LI

期刊论文

Emissions analysis on second generation biodiesel

Yuvarajan Devarajan,Ravi kumar Jayabal,Devanathan Ragupathy,Harish Venu

期刊论文

Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division

Claudia DOEL,Zhidong ZHANG,Lise MAZELET,Ryan WATERS,John BASHIRUDDIN

期刊论文

The second short-term warm ischemia after vascular anastomosis did not affect early renal function recovery

null

期刊论文

Second unmanipulated allogeneic transplantation could be used as a salvage option for patients with relapsed

期刊论文

Development of splenic surgery in China

JIANG Hongchi, ZHAO Xianqi, GAO Yue

期刊论文

分形方法导出改进的牛顿第二定律及万有引力定律

付昱华

期刊论文

Comparison between linear and non-linear forms of pseudo-first-order and pseudo-second-order adsorption

Junxiong LIN , Lan WANG ,

期刊论文